Disclosed are a secondary battery comprising a negative electrode composed of two or more negative electrode plates and a method of manufacturing the secondary battery, wherein each of the negative electrode plates includes a lithium by-product layer formed through pre-lithiation reaction on a negative electrode current collector coated with a negative electrode active material, wherein an inorganic substance layer is formed on a negative electrode tab that is extended from an end at one side of the negative electrode current collector and is composed of an active material-non-coated portion not coated with the negative electrode active material, and negative electrode tabs of the negative electrode plates are electrically connected with one negative electrode lead to form a negative electrode terminal.