A method of handling signal transmission applicable to a timing controller of a display system, wherein the timing controller is couplable to at least one source driver of the display system via a plurality of mini low voltage differential signaling (mini-LVDS) channels, includes transmitting a reset signal to each of the at least one source driver via a first mini-LVDS channel among the plurality of mini-LVDS channels; generating a first control signal for setting the at least one source driver; and transmitting the first control signal to each of the at least one source driver via a second mini-LVDS channel among the plurality of mini-LVDS channels when the reset signal is transmitted via the first mini-LVDS channel.