A parallax barrier, a display device and a manufacturing method thereof are disclosed. The parallax barrier includes a plurality of strip-shaped polarizing portions and a plurality of strip-shaped transparent portions, the plurality of strip-shaped polarizing portions and the plurality of strip-shaped transparent portions extend in the same direction, and the plurality of strip-shaped polarizing portions and the plurality of strip-shaped transparent portions are alternately arranged. With the parallax barrier, naked eye or multi-view display can be achieved by light splitting, and the curing non-uniformity in the curing and bonding process can be alleviate to increase the manufacturing yield.