A testing method for splicing screens is provided. The method includes: outputting an image signal to a display screen, wherein the display screen includes a plurality of splicing screens, the image signal includes a plurality of testing sub pictures, the testing sub pictures are arranged correspondingly to the splicing screens so as to enable the splicing screens to display the corresponding testing sub pictures, and the testing sub pictures include testing contents; and testing the splicing screens through the testing contents, wherein the testing contents include a graduation signal, by which an accurate test is performed on the splicing screens.