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US10669308B2 Method capable of being used for protein renaturation independently or being used as preceding operations of protein renaturation 审中-公开

  • 专利标题： Method capable of being used for protein renaturation independently or being used as preceding operations of protein renaturation
  • 申请号： US15840024
    申请日： 2017-12-13
  • 公开(公告)号： US10669308B2
    公开(公告)日： 2020-06-02
  • 发明人: Peng Zhang
  • 申请人： Peng Zhang
  • 代理机构： Hodgson Russ LLP
  • 主分类号： C07K1/30
  • IPC分类号： C07K1/30 ;  C07K1/113
Method capable of being used for protein renaturation independently or being used as preceding operations of protein renaturation
摘要：
Provided is a method of treating a protein to be renatured, including: (1) mixing the protein to be renatured with a denaturing solution containing a denaturing reagent; (2) incubating a resulting mixture at such a low temperature that the denaturing agent is gradually precipitated from the denaturing solution, resulting in a decreasing concentration gradient of the denaturing agent and an increasing concentration of a renatured protein or its precursor in the denaturing solution with a decreasing volume; and (3) obtaining at least one of the renatured protein and its precursor.
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