A 14C testing bottle, a 14C testing device, a 14C testing method, a sampling and preparation system and its implementation method are provided. The 14C testing bottle includes a pressure-bearing shell and a sample bin positioned in the pressure-bearing shell. A cavity is arranged in the sample bin and the 14C testing bottle is provided with an injection port connected to the cavity. The sample bin may diffuse the light produced in the cavity and at least part of the sample bin is transparent. An optical fiber channel is set on the pressure-bearing shell. One end of the optical fiber channel is connected with an external scintillation counter, and the other end of the optical fiber channel is connected with the transparent part of the sample bin. The 14C testing bottle may measure the 14C content in the carbon dioxide sample rapidly.