Provided herein is a method of manufacturing a substrate for a display device, the method including: preparing a supporting substrate having a first surface and a second surface opposite to the first surface; patterning a blocking film on the first surface of the supporting substrate; forming a substrate layer on the supporting substrate to cover the blocking film; removing at least a portion of the substrate layer to space the blocking film apart from the substrate layer by a predetermined distance; and separating the substrate layer from the supporting substrate.