Invention Grant
- Patent Title: Methods and compositions for generating epicardium cells
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Application No.: US14915992Application Date: 2014-09-12
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Publication No.: US10711246B2Publication Date: 2020-07-14
- Inventor: Gordon Keller , Alec Drake Witty , Steven James Kattman
- Applicant: UNIVERSITY HEALTH NETWORK
- Applicant Address: CA Toronto, Ontario
- Assignee: UNIVERSITY HEALTH NETWORK
- Current Assignee: UNIVERSITY HEALTH NETWORK
- Current Assignee Address: CA Toronto, Ontario
- Agency: Proskauer Rose LLP
- International Application: PCT/CA2014/000687 WO 20140912
- International Announcement: WO2015/035506 WO 20150319
- Main IPC: C12N5/077
- IPC: C12N5/077 ; C12Q1/6881 ; G01N33/50

Abstract:
Provided are methods and products for obtaining cardiovascular lineage cells from hPSCs. The method for obtaining a cardiomyocyte lineage or an epicardial lineage cell population from human pluripotent stem cells (hPSCs) comprises one or more of the following steps: (a) contacting BMP component primed hPSCs with a cardiovascular mesoderm programming cocktail suitable for inducing the hPSCs to differentiate to a cardiovascular mesoderm cell population under conditions suitable for the programming cocktail to penetrate the hPSCs and culturing the contacted hPSCs for a period of time to generate a KDR+ and PDGFRalpha+ cardiovascular mesoderm cell population; (b) contacting the cardiovascular mesoderm cell population with a cardiovascular progenitor specification cocktail suitable to specify a NKX2-5+ or WT1+ cardiovascular progenitor cell population under conditions suitable for the specification cocktail to penetrate the cardiovascular mesoderm cell population and culturing the contacted cardiovascular mesoderm cell population for a period of time to generate a NKX2-5+ or WT1+ cardiovascular progenitor cell population; and (d) contacting the cardiovascular progenitor cell population with a maturation cocktail under conditions suitable for the maturation cocktail to penetrate the cardiovascular progenitor cell population and culturing the contacted cardiovascular progenitor population for a period of time to produce a cardiovascular population optionally cardiomyocyte lineage cells expressing cardiac troponin T (cTnT) and/or SIRPA and/or epicardial lineage cells expressing WT1.
Public/Granted literature
- US20160215263A1 METHODS AND COMPOSITIONS FOR GENERATING EPICARDIUM CELLS Public/Granted day:2016-07-28
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