A driving method of a display panel is provided, including setting the first red, first green, first blue, second red, second green, and second blue multiplexed signals. In the 2i−1th multiplexing period, the charging time of the sub-pixels corresponding to the switching units controlled by the first red, the first green, and the first blue multiplexed signals is earlier than that controlled by the second red, the second green, and the second blue multiplexed signals. While In the 2ith multiplexing period, the charging time of the sub-pixels corresponding to the switching units controlled by the first red, the first green, and the first blue multiplexed signals is later than that controlled by the second red, the second green, and the second blue multiplexed signals. The method will eliminate the stripe feeling of the scream picture displayed on the display panel and so improve the display quality.