An automatic operating device for hollow fiber membrane includes a first membrane fiber traction apparatus, a membrane fiber bracket member for fixing the membrane fiber, and a second fiber traction apparatus, which are disposed in sequence. The membrane fiber bracket member comprises a first membrane fiber bracket and a second membrane fiber bracket each having upper openings. The first membrane fiber traction apparatus, the first membrane fiber bracket, the second membrane fiber bracket, and the second membrane fiber traction apparatus are all provided with membrane fiber holes matched with the membrane fiber. The automatic operating device further comprises a membrane fiber grabbing mechanism, a first driving mechanism, a cutting mechanism and a glue-filling mechanism. The present invention can realize the automatic penetration of membrane fiber into the membrane fiber brackets and the traction apparatus, and realize formation of the membrane fiber in a regular arrangement.