A system for light source calibration and aging compensation is disclosed. In one or more embodiments, the system includes a light source driver, a sensor, and a controller. The light source driver is configured to generate one or more power signals for a plurality of light sources. The sensor is configured to detect an illumination power output by at least one light source of the plurality of light sources. The controller is communicatively coupled to the sensor and the light source driver. The controller is configured to compare the illumination power detected by the sensor to a target illumination power. The controller is further configured to generate one or more control signals to adjust an electrical power output of the light source driver based on the comparison of the illumination power detected by the sensor to the target illumination power.