A system and method for calibrating an acoustic monitoring system of an additive manufacturing machine includes installing a calibration system on the machine and performing a calibration process. Specifically, the calibration system includes a calibration platform removably mountable to a build platform of the additive manufacturing machine and having calibrated acoustic source mounted thereon for defining a measurement standard. The acoustic waves generated by the calibrated acoustic source are measured by the acoustic monitoring system and compared to the known measurement standard to determine whether system adjustments would improve process tolerances or uniformity.