The present disclosure provides a display panel, a manufacturing method thereof, and a display device, where the display panel includes a first substrate, a second substrate, a first spacer, and a second spacer. The first substrate and the second substrate are disposed opposite to each other. The first spacer is disposed on a side of the first substrate adjacent to the second substrate. The second spacer is disposed on a side of the second substrate adjacent to the first substrate. The first spacer and the second spacer are butted with each other through one of two embeddings in the other.