A light source unit includes: a first light emitting element configured to emit a first wavelength band light; a second light emitting element configured to emit a second wavelength band light; a luminescent wheel configured to have a first fluorescent light emitting region irradiated with the second wavelength band light to emit a third wavelength band light, and a second fluorescent light emitting region that emits a fourth wavelength band light including a wavelength band of the first wavelength band light and the third wavelength band light having a wavelength band adjacent to the first wavelength band light being provided in parallel in a circumferential direction; a color wheel configured to have a second transmissive region that transmits the first wavelength band light to the fourth wavelength band light, and a third transmissive region that transmits only the first wavelength band light or transmits only the first wavelength band light and the second wavelength band light being installed in parallel in the circumferential direction, and synchronously rotate with the luminescent wheel; and a controller configured to cause the second wavelength band light to be emitted in each output period and the first wavelength band light to be emitted in the output period in which the fourth wavelength band light is emitted, in a plurality of output periods in a frame.