A laboratory tracer experiment system based on karst conduit medium characteristic inversion, having: a assembly model system of the karst conduit medium; a test bed, configured to support the assembly model system of the karst conduit medium; a water supply system connected to the assembly model system of the karst conduit medium to supply water to the assembly model system of the karst conduit medium; a full-automatic control system for tracer adding connected to the assembly model system of the karst conduit medium to add a prepared tracer solution into the assembly model system of the karst conduit medium; a real-time wireless monitoring system of fluorescent tracer; and a central control system for controlling the full-automatic control system for tracer adding, the water supply system, the real-time wireless monitoring system of fluorescent tracer and the high-definition camera recording system to communicate with the central control system.