- 专利标题: Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
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申请号: US15529597申请日: 2015-12-08
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公开(公告)号: US11008604B2公开(公告)日: 2021-05-18
- 发明人: Kathryn Louise Lamerton , Jeffrey Kenneth Horton , Peter James Tatnell
- 申请人: GE HEALTHCARE UK LIMITED
- 申请人地址: GB Little Chalfont
- 专利权人: GE HEALTHCARE UK LIMITED
- 当前专利权人: GE HEALTHCARE UK LIMITED
- 当前专利权人地址: GB Little Chalfont
- 代理机构: Eversheds Sutherland (US) LLP
- 国际申请: PCT/US2015/064409 WO 20151208
- 国际公布: WO2016/099999 WO 20160623
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N33/50 ; G01N33/53 ; C12Q1/6804 ; G01N33/543 ; C12Q1/686 ; C12Q1/6851
摘要:
Embodiments of the invention provides a method for detection of at least one analyte derived from a sample, comprising the steps of: a) depositing the sample on a surface of a solid support; b) transferring at least a portion of the solid support to a receptacle suitable for performing a specific binding assay for one or more analytes of interest; c) optionally washing the portion; d) adding a single specific binding partner for each analyte of interest to the receptacle, the binding partner being labelled with an oligonucleotide sequence; e) mixing the portion with nucleic acid amplification reagents; f) amplifying the oligonucleotide sequence; and g) detecting amplified nucleic acid. The invention also provides a kit for use with the method for detection of at least one analyte derived from a sample.
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