An autofocus control device includes a processor. The processor performs an image acquisition process of acquiring an object image captured by an imaging section, a treatment determination process of determining whether or not treatment is being performed based on the object image, a focus evaluation area setting process of setting either a preset area or a treatment area set based on the object image, as a focus evaluation area in the object image based on a determination result of the treatment determination process, and a focus control process of performing focus control of the imaging section based on a focus evaluation value of the focus evaluation area.