Disclosed is a method and apparatus for transmitting and receiving a Reference Signal (RS) performed by a User Equipment (UE) in a wireless communication system. According to the present invention, a method and apparatus includes receiving a first Dedicated Demodulation Reference Signal and a first Physical Downlink Shared Channel (PDSCH); receiving a Physical Downlink Control Channel (PDCCH) including Downlink Control Information (DCI) in relation to a configuration of the second Dedicated Demodulation Reference Signal based on the first PDSCH; and receiving the second Dedicated Demodulation Reference Signal and a second PDSCH based on the PDCCH, and the first Dedicated Demodulation Reference Signal is transmitted only on a single antenna port, and a configuration of the DCI is determined based on the maximum number of symbols to which the second Dedicated Demodulation Reference Signal is mapped.