- 专利标题： Phytase variants YeAPPA having improved gastric protein resistance and acid resistance, and increased catalytic efficiency
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申请号： US16477903申请日： 2018-01-15
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公开(公告)号： US11155793B2公开(公告)日： 2021-10-26
- 发明人: Canfang Niu , Peilong Yang , Bin Yao , Yangyang Li , Yongkai Du , Huiying Luo , Huoqing Huang , Yaru Wang
- 申请人： FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICUL , Canfang Niu , Peilong Yang , Bin Yao , Yangyang Li , Yongkai Du , Huiying Luo , Huoqing Huang , Yaru Wang
- 申请人地址： CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing
- 专利权人： FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICUL,Canfang Niu,Peilong Yang,Bin Yao,Yangyang Li,Yongkai Du,Huiying Luo,Huoqing Huang,Yaru Wang
- 当前专利权人： FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICUL,Canfang Niu,Peilong Yang,Bin Yao,Yangyang Li,Yongkai Du,Huiying Luo,Huoqing Huang,Yaru Wang
- 当前专利权人地址： CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing; CN Beijing
- 代理机构： Patshegen IP LLC
- 代理商 Moshe Pinchas
- 优先权： CN201710027074.8 20170115
- 国际申请： PCT/CN2018/072540 WO 20180115
- 国际公布： WO2018/130211 WO 20180719
- 主分类号： C12N9/16
- IPC分类号： C12N9/16 ; C12N1/21 ; C12N15/55 ; A23K20/189 ; C12N15/52 ; C12N15/70
摘要：
The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162th site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230th site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.
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