公开(公告)号：US20100092613A1

公开(公告)日：2010-04-15

申请号：US12298948

申请日：2006-04-30

申请人： Bin Yao ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang ,  Tiezheng Yuan ,  Xiuyun Shi ,  Yingguo Bai ,  Kun Meng ,  Peilong Yang

发明人： Bin Yao ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang ,  Tiezheng Yuan ,  Xiuyun Shi ,  Yingguo Bai ,  Kun Meng ,  Peilong Yang

IPC分类号： A23K1/165 ,  C12N9/16 ,  C12N15/00 ,  C12N1/19 ,  C12N1/20 ,  C12P21/02 ,  C12P19/34

CPC分类号： C07K14/195 ,  A23K20/189 ,  A23K50/00 ,  C12N9/16 ,  Y10S530/825

摘要： The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novel Yersinia intermedia having phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960±248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60° C., (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g., Pichia pastoris) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient. In addition, the present invention provides a novel method for isolating phytase from a target organism.

摘要翻译： 本发明涉及一种新的植酸酶，一种编码该酶的新的分离的核酸分子，以及具有植酸酶活性的新颖的中间叶耶尔森菌。 特别地，本发明涉及具有（a）理论分子量45.5kDa，（b）高比活性3960±248U / mg的植酸酶，（c）在高温和宽pH下的高稳定性，（d）最佳pH 4.0-5.0，（e）50-60℃的最佳温度，（f）对胃蛋白酶和胰蛋白酶的高抗性。 植酸酶非常适合用作饲料添加剂的单胃饲料。 本发明还涉及包含所述核酸分子的重组载体，携带所述重组载体的重组宿主细胞（例如巴斯德毕赤酵母），以及使用重组宿主细胞产生肌醇六磷酸酶的方法。 本发明还提供一种饲料添加剂，其包含表达植酸酶作为有效成分的所述植酸酶和/或宿主细胞。 此外，本发明提供了从目标生物体中分离植酸酶的新方法。

公开(公告)号：US08455620B2

公开(公告)日：2013-06-04

申请号：US12298948

申请日：2006-04-30

申请人： Bin Yao ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang ,  Tiezheng Yuan ,  Xiuyun Shi ,  Yingguo Bai ,  Kun Meng ,  Peilong Yang

发明人： Bin Yao ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang ,  Tiezheng Yuan ,  Xiuyun Shi ,  Yingguo Bai ,  Kun Meng ,  Peilong Yang

IPC分类号： C07K14/195

CPC分类号： C07K14/195 ,  A23K20/189 ,  A23K50/00 ,  C12N9/16 ,  Y10S530/825

摘要： The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novel Yersinia intermedia having phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960±248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60° C., (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g., Pichia pastoris) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient. In addition, the present invention provides a novel method for isolating phytase from a target organism.

摘要翻译： 本发明涉及一种新的植酸酶，一种编码该酶的新的分离的核酸分子，以及具有植酸酶活性的新颖的中间叶耶尔森菌。 特别地，本发明涉及具有（a）理论分子量45.5kDa，（b）高比活性3960±248U / mg的植酸酶，（c）在高温和宽pH下的高稳定性，（d）最佳pH 4.0-5.0，（e）50-60℃的最佳温度，（f）对胃蛋白酶和胰蛋白酶的高抗性。 植酸酶非常适合用作饲料添加剂的单胃饲料。 本发明还涉及包含所述核酸分子的重组载体，携带所述重组载体的重组宿主细胞（例如巴斯德毕赤酵母），以及使用重组宿主细胞产生肌醇六磷酸酶的方法。 本发明还提供一种饲料添加剂，其包含表达植酸酶作为有效成分的所述植酸酶和/或宿主细胞。 此外，本发明提供了从目标生物体中分离植酸酶的新方法。

公开(公告)号：US11155793B2

公开(公告)日：2021-10-26

申请号：US16477903

申请日：2018-01-15

申请人： FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICUL ,  Canfang Niu ,  Peilong Yang ,  Bin Yao ,  Yangyang Li ,  Yongkai Du ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang

发明人： Canfang Niu ,  Peilong Yang ,  Bin Yao ,  Yangyang Li ,  Yongkai Du ,  Huiying Luo ,  Huoqing Huang ,  Yaru Wang

IPC分类号： C12N9/16 ,  C12N1/21 ,  C12N15/55 ,  A23K20/189 ,  C12N15/52 ,  C12N15/70

摘要： The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162th site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230th site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.