A three-piece locking fastener including an interiorly threaded intermediate component open at opposite ends. A first screw having a first exterior thread pattern rotationally inter-engages with the interior threads of the intermediate component when installed through a first selected one of the opposite ends, the first screw having a hollow shaft exhibiting a further plurality of interior threads matching a direction of the first thread pattern. A second screw has a second exterior thread pattern opposite the first pattern and, upon being installed within the other selected one of the opposite ends, rotationally inter-engages the interior threads of the first screw, so that loosening of either of the first and second screws being prevented by their counter-threaded orientation.