- 专利标题: Genotyping of mutations by combination of in-tube hybridization and universal tag-microarray
-
申请号: US16493607申请日: 2018-03-30
-
公开(公告)号: US11230732B2公开(公告)日: 2022-01-25
- 发明人: Marcella Chiari , Francesco Damin , Silvia Galbiati , Maurizio Ferrari
- 申请人: Marcella Chiari , Francesco Damin , Silvia Galbiati , Maurizio Ferrari
- 申请人地址: IT Milan; IT Busto Arsizio; IT Cernusco sul Naviglio; IT Milan
- 专利权人: Marcella Chiari,Francesco Damin,Silvia Galbiati,Maurizio Ferrari
- 当前专利权人: Marcella Chiari,Francesco Damin,Silvia Galbiati,Maurizio Ferrari
- 当前专利权人地址: IT Milan; IT Busto Arsizio; IT Cernusco sul Naviglio; IT Milan
- 代理机构: The Marbury Law Group, PLLC
- 国际申请: PCT/IB2018/052219 WO 20180330
- 国际公布: WO2018/178943 WO 20181004
- 主分类号: C12Q1/6837
- IPC分类号: C12Q1/6837 ; C12Q1/6827
摘要:
An assay for detecting gene fragments, including: amplifying gene fragments comprising single-nucleotide polymorphisms (SNPs) to form an initial amplification product; isolating single strand oligonucleotides of interest from the initial amplification product; forming a solution comprising the oligonucleotides of interest and reporter molecules, each reporter molecule having a first oligonucleotide domain configured to hybridize with a complementary oligonucleotide of interest, and a second oligonucleotide domain configured to hybridize with a complementary capture probe; hybridizing, in the solution, the oligonucleotides of interest with the reporter molecules that have complementary first domains; applying the solution to the surface of a microarray including an array of capture probes fixed to a microarray slide; capturing the oligonucleotides of interest on the microarray by hybridizing the second oligonucleotide domains of the reporter molecules with complementary capture probes of the microarray; and detecting the hybridized oligonucleotides of interest captured on the microarray.
信息查询
IPC分类: