The present disclosure relates to the field of stroke-controlling mechanism, and discloses a stroke-limiting control assembly and an operating method. The stroke-limiting control assembly comprises a housing and a film winding. A transmission input gear, a transmission gear, a guide wheel, and a spring box that can be rotated to reset by an elastic force are rotatably connected within the housing. When a moving component moves to an end of an upper stroke, a first metal needle abuts a first proximity switch. When the movable component moves to an end of a lower stroke, a second metal needle abuts against a second proximity switch. At least a stroke-limiting control assembly is provided which enables simplification of a product requiring stroke-limiting, such as an electric jack or an electric support leg, by arranging the proximity switches outside a screw nut lifting unit in the electric jack or the electric support leg.