- 专利标题: Microscopic imaging method using a correction factor
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申请号: US16445802申请日: 2019-06-19
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公开(公告)号: US11238575B2公开(公告)日: 2022-02-01
- 发明人: Viktor Drescher , Nils Langholz
- 申请人: Carl Zeiss Microscopy GmbH
- 申请人地址: DE Jena
- 专利权人: Carl Zeiss Microscopy GmbH
- 当前专利权人: Carl Zeiss Microscopy GmbH
- 当前专利权人地址: DE Jena
- 代理机构: Duane Morris LLP
- 优先权: DE102018210606.1 20180628
- 主分类号: G06T5/50
- IPC分类号: G06T5/50 ; H04N5/225 ; H04N5/232 ; G02B21/00 ; G06T5/00 ; H04N5/32 ; G06T7/00
摘要:
A microscopic imaging method, includes illuminating a specimen with illumination radiation and capturing detection radiation along a detection axis. The detection radiation is caused by the illumination radiation, at a first time as a wide-field signal and at a second time as a composite signal. The composite signal is formed by a superposition of a confocal image and a wide-field image; extracting the confocal image by subtracting the wide-field signal from the composite signal, wherein a correction factor is used. A current correction factor is ascertained for each executed imaging and/or for each imaged specimen (1) and the confocal image is extracted using the respective current correction factor.
公开/授权文献
- US20200005442A1 MICROSCOPIC IMAGING METHOD USING A CORRECTION FACTOR 公开/授权日:2020-01-02
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