- 专利标题: Method for producing cellulase and apparatus for said method
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申请号: US14359122申请日: 2012-11-21
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公开(公告)号: US11253818B2公开(公告)日: 2022-02-22
- 发明人: Hiroyuki Kurihara , Katsushige Yamada
- 申请人: Toray Industries, Inc.
- 申请人地址: JP Tokyo-to
- 专利权人: Toray Industries, Inc.
- 当前专利权人: Toray Industries, Inc.
- 当前专利权人地址: JP Tokyo-to
- 代理机构: DLA Piper LLP (US)
- 优先权: JPJP2011-253706 20111121
- 国际申请: PCT/JP2012/080123 WO 20121121
- 国际公布: WO2013/077341 WO 20130530
- 主分类号: B01D61/18
- IPC分类号: B01D61/18 ; C12M1/00 ; C12P21/00 ; B01D61/16 ; B01D61/14 ; C12N9/42
摘要:
A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.
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