An air suspension support and motion guiding device having unequal-depth throttling chambers. Throttling plugs (10) are arranged regularly on an air suspension working surface, forming an array of throttling chambers with unequal depths. The invention improves the rotational stiffness per unit area for an air suspension working surface while ensuring support and stability of an air-suspended rail (1) and enables construction of a small-volume, long cantilever, high torque load two-dimensional motion reference device.