Provided are a service transmission method and device using a FlexO, equipment and a storage medium. The method includes: customer service data is mapped into N FlexO frames on M Physical Layer links of a FlexO transmission group, and then the N FlexO frames are sent through the FlexO transmission group; and a receiving end sequentially extracts the customer service data from the N FlexO frames. The FlexO transmission group comprises M PHY links. The customer service data occupies the same number of cells in the FlexO frame of each PHY link, and cell locations of the occupied cells are the same. A set of logic is used to directly map the customer service data into the N FlexO frames on the M PHY links of the FlexO transmission group, so as to minimize complexity and logical resources needing to be occupied.