Invention Grant
- Patent Title: Viridiflorol production in auxotrophic Escherichia coli
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Application No.: US16931711Application Date: 2020-07-17
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Publication No.: US11332765B2Publication Date: 2022-05-17
- Inventor: Congqiang Zhang , Xixian Chen , Sudha Shukal
- Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
- Applicant Address: SG Singapore
- Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
- Current Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
- Current Assignee Address: SG Singapore
- Agency: Marshall, Gerstein & Borun LLP
- Priority: SG10201907354Y 20190808
- Main IPC: C12P15/00
- IPC: C12P15/00 ; C12N9/88 ; C12N15/70

Abstract:
A method of increasing terpenoid production in a host cell that produces one or more terpenoids, comprising: a) providing a host cell that produces one or more terpenoids, said host cell comprising a vector comprising a polynucleotide sequence encoding a terpene synthase enzyme; b) modifying the vector to:
i. introduce an inducible promoter operably linked to the polynucleotide sequence encoding the terpene synthase enzyme; and ii. introduce a polynucleotide sequence encoding a ribosomal binding site (RBS) that increases translation initiation rate of the terpenoid compared to a wild type ribosomal binding site; c) determining the dosage of an inducer capable of inducing the inducible promoter; d) culturing the host cell in a culture medium in the presence of the inducer at the dosage determined from step c); and e) isolating the terpenoid from the culture medium.
i. introduce an inducible promoter operably linked to the polynucleotide sequence encoding the terpene synthase enzyme; and ii. introduce a polynucleotide sequence encoding a ribosomal binding site (RBS) that increases translation initiation rate of the terpenoid compared to a wild type ribosomal binding site; c) determining the dosage of an inducer capable of inducing the inducible promoter; d) culturing the host cell in a culture medium in the presence of the inducer at the dosage determined from step c); and e) isolating the terpenoid from the culture medium.
Public/Granted literature
- US20210040520A1 VIRIDIFLOROL PRODUCTION IN AUXOTROPHIC ESCHERICHIA COLI Public/Granted day:2021-02-11
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