The present disclosure provides a display substrate which includes: a base substrate; a light emitting layer located on the base substrate, the light emitting layer including light emitting regions and non-light emitting regions which are arranged alternately, the light emitting regions including multiple light emitting units; and the display substrate further includes a fingerprint recognition region including a touch layer which is disposed on a side of the light emitting layer distal to the base substrate and corresponds to the non-light emitting regions of the light emitting layer, the touch layer including an opaque pattern; the touch layer is provided with at least one pinhole.