A display substrate, includes: a plurality of pixel driving circuits; a plurality of groups of light-emitting driving signal lines, wherein each driving signal line group of the plurality of groups of light-emitting driving signal lines includes a plurality of light-emitting driving signal lines; and a plurality of pixel circuit multiplexing units coupled to the plurality of pixel driving circuits, respectively, wherein each pixel circuit multiplexing unit includes N light-emitting units coupled to one of the plurality of pixel driving circuits and a group of light-emitting driving signal line.