Micro-fluidic chip comprises substrate and plurality of driving circuits on substrate, each of plurality of driving circuits comprising: driving electrode comprising first electrode plate and second electrode plate made of different materials on substrate, first electrode plate being electrically coupled to second electrode plate; and detecting sub-circuit comprising first signal terminal electrically coupled to first electrode plate and second signal terminal electrically coupled to second electrode plate, wherein micro-fluidic chip further comprises: voltage supply sub-circuit configured to supply driving voltage to first signal terminal to control droplet to move toward driving circuit during droplet driving stage, and configured to supply constant voltage to first signal terminal, during temperature detecting stage, and wherein detecting sub-circuit is configured to measure voltage difference between first signal terminal and second signal terminal, and obtain temperature of droplet on second electrode plate according to voltage difference, during temperature detecting stage.