Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.