The present disclosure provides a display substrate and a manufacturing method thereof, a display panel and a manufacturing method and a driving method thereof, and a display apparatus. The display substrate includes: a base substrate; and a first electrode, a signal line, and an organic layer between the first electrode and the signal line, all of which are on the base substrate, wherein the display substrate further comprises a conductive pillar configured to penetrate the organic layer and electrically connect the first electrode to the signal line.