Invention Grant
- Patent Title: Method for processing rolling circle amplification products
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Application No.: US16987283Application Date: 2020-08-06
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Publication No.: US11365440B2Publication Date: 2022-06-21
- Inventor: Mathias Howell , Ove Öhman , Fredrik Persson , Linus Olausson
- Applicant: Vanadis Diagnostics
- Applicant Address: SE Sollentuna
- Assignee: Vanadis Diagnostics
- Current Assignee: Vanadis Diagnostics
- Current Assignee Address: SE Sollentuna
- Agency: Bozicevic, Field & Francis LLP
- Agent James S. Keddie
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/682 ; C12Q1/6851 ; B01J13/04 ; C08L83/04 ; G01N33/58

Abstract:
This disclosure provides, among other things, a method for processing a membrane comprising rolling circle amplification (RCA) products. In some embodiments, this method may comprise: (a) obtaining a porous capillary membrane that comprises fluorescently labeled RCA products that are in or on the membrane; (b) depositing a curable polymer onto the membrane; and (c) curing the curable polymer to encapsulate the RCA products in a solid. In some embodiments, the curable polymer may be a silicone and may be transparent in its solid form. A kit for performing the method and a composition made by the method are also provided.
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