The present invention belongs to mosquito repellent technical field, and specifically a mosquito repellent composition, preparation method and application thereof. The mosquito repellent composition comprises following ingredients and mass fractions thereof: composite plant oil 7-10 parts, gromwell root extracts 3-5 parts, Vitex rotundifolia extracts 1-3 parts, sustained release agents 1-2 parts, emulsifying agents 2-4 parts, stabilizing agents 0.1-0.3 part, and water 165-182 parts. The mosquito repellent composition provided in the present invention doesn't contain chemical ingredients such as DEET and methothrin etc., and works in combination with natural plant essence oil, which is highly safe, free from irritation, allergic reactions and other side effects, and can be used to repel mosquitoes safely, gently and is suitable for long term use.