The invention discloses a three-dimensional display module using optical wave-guide for providing directional backlights. This display module includes a wave-guide backlight unit with time-sequential directional light sources, a display device, a wavefront modulation device or a light splitting device, and other components. The wave-guide backlight unit with time-sequential directional light sources includes a sequential-switching light-source array, a relay device, and an optical wave-guide device. Each light source of the sequential-switching light-source array provides backlight of corresponding vector characteristics to the display device, making optical message displayed by the display device being guided to the corresponding viewing zone/viewing zones. Through the technology routes of multiple-view-for-one-pupil or/and Maxwellian view, the three-dimensional display module implements displays free from the vergence-accommodation conflict. The introduction of the optical wave-guide device results in a thin structure, and the disclosed display module can be applied to various display terminals, such as mobile phones, iPads, head-mounted VR/AR, etc.