A door opening and closing device for vehicles of the present invention includes: a motor drive mechanism; a first housing that accommodates the motor drive mechanism; a spindle drive mechanism connected to the motor drive mechanism; and a second housing that is arranged coaxially with the first housing and relatively moves with respect to the first housing by the spindle drive mechanism. The spindle drive mechanism includes: a spindle connected to the motor drive mechanism; a spindle nut screwed with the spindle; a push rod fixed so as not to rotate relative to the spindle nut and provided with a protrusion on its outer periphery; and a guide tube in which a guide groove guiding the protrusion of the push rod in an axial direction is formed.