- 专利标题： Methods for disinfecting and inducing direct rapid proliferation of explants of Kadsura coccinea stems with buds
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申请号： US17295035申请日： 2020-06-04
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公开(公告)号： US11547071B2公开(公告)日： 2023-01-10
- 发明人: Ze Li , Yang Liu , Hui Zhang , Xiaoqin Zhang , Xiaohui Gao , Fangfang Ma , Jiayue Zhong , Xiaoyan Zhang , Sen Wang , Xiaofeng Tan , Ao Yang , Fen Bao , Ruonan Ma
- 申请人： CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
- 申请人地址： CN Changsha
- 专利权人： CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
- 当前专利权人： CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
- 当前专利权人地址： CN Changsha
- 代理机构： Bayramoglu Law Offices LLC
- 优先权： CN201911012181.9 20191023
- 国际申请： PCT/CN2020/094369 WO 20200604
- 国际公布： WO2021/077755 WO 20210429
- 主分类号： A01H4/00
- IPC分类号： A01H4/00 ; A01G2/10 ; A01G24/22 ; A01G7/06 ; A01G23/04
摘要：
A method for disinfecting explants of Kadsura coccinea stems with buds and a method for directly inducing rapid proliferation of sterile buds by using the explants of Kadsura coccinea stems with buds involve processes such as selection, treatment and disinfection of explants, primary culture, subculture proliferation culture. The problem of difficulty in tissue culture and primary culture of Kadsura coccinea stems is solved, and the advantages include low contamination rate of explants, high propagation rate and robust proliferation of axillary buds. This allows obtaining sterile axillary buds of Kadsura coccinea through tissue culture, and provides support for tissue culture, rapid propagation and factory seedling of Kadsura coccinea in the future.
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