公开(公告)号：US11547071B2

公开(公告)日：2023-01-10

申请号：US17295035

申请日：2020-06-04

申请人： CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

发明人： Ze Li ,  Yang Liu ,  Hui Zhang ,  Xiaoqin Zhang ,  Xiaohui Gao ,  Fangfang Ma ,  Jiayue Zhong ,  Xiaoyan Zhang ,  Sen Wang ,  Xiaofeng Tan ,  Ao Yang ,  Fen Bao ,  Ruonan Ma

IPC分类号： A01H4/00 ,  A01G2/10 ,  A01G24/22 ,  A01G7/06 ,  A01G23/04

摘要： A method for disinfecting explants of Kadsura coccinea stems with buds and a method for directly inducing rapid proliferation of sterile buds by using the explants of Kadsura coccinea stems with buds involve processes such as selection, treatment and disinfection of explants, primary culture, subculture proliferation culture. The problem of difficulty in tissue culture and primary culture of Kadsura coccinea stems is solved, and the advantages include low contamination rate of explants, high propagation rate and robust proliferation of axillary buds. This allows obtaining sterile axillary buds of Kadsura coccinea through tissue culture, and provides support for tissue culture, rapid propagation and factory seedling of Kadsura coccinea in the future.