The display substrate includes: the flexible base substrate and the trace layer, a first adhesion-enhancing layer, and a first insulating layer that are stacked on the flexible base substrate, wherein the first adhesion-enhancing layer is between the first insulating layer and the trace layer, and the first adhesion-enhancing layer is bonded to the first insulating layer and the trace layer respectively. The flexible base substrate is provided with a bending area, and an overlapping area is present between an area which an orthographic projection of the first adhesion-enhancing layer on the flexible base substrate is within and the bending area, and an orthographic projection of the signal trace in the trace layer on the flexible base substrate falls in the bending area.