Provided is a method for berth allocation of a multiline bus station and speed guidance of buses. The intelligent control center sorts all buses between the current station and the previous station to form the BusNumber table, and performs speed guidance for all buses in the BusNumber table. Whether the guided bus can pass through the signalized intersection is determined. The buses that cannot pass through the signalized intersection are guided by acceleration, and those that cannot pass through the signalized intersection by acceleration guidance method update their speeds based on the phase state of signal light, so as to pass through the signalized intersection without stopping in most cases. All buses are conducted through speed guidance methods, and the berths are allocated for all the buses under different conditions.