Provided is a display substrate including: a base substrate, a plurality of micro light-emitting diodes and a plurality of touch electrodes; wherein the micro light-emitting diode comprises: a first electrode, a light-emitting layer, and a second electrode that are sequentially arranged in a direction distal from the base substrate; and the touch electrode is disposed on a side of the micro LED distal from the base substrate. A manufacturing method of manufacturing a display substrate, a display panel, and a display device are also provided.