Devices and methods in which auscultation signals, ultrasound signals, and ambient noise signals may be simultaneously acquired by a same handheld device are provided. Moreover, in some embodiments, electrocardiogram (EKG) may be acquired by the handheld device. One such device includes a housing having a sensor portion at a distal end of the housing, and a handle portion between a proximal end and the distal end of the housing. An ultrasound sensor is positioned at least partially within the sensor portion of the housing, and a first auscultation sensor is positioned at least partially within the sensor portion of the housing. An ambient noise sensor is positioned at least partially within the housing between the handle portion and the proximal end of the housing.