Invention Grant
- Patent Title: Sirtuin 4 (SIRT4) lipoamidase activity and uses thereof
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Application No.: US16991429Application Date: 2020-08-12
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Publication No.: US11726088B2Publication Date: 2023-08-15
- Inventor: Thomas Shenk , Todd M. Greco , Ileana M. Cristea , Rommel A. Mathias , Adam Oberstein
- Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
- Applicant Address: US NJ Princeton
- Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
- Current Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
- Current Assignee Address: US NJ Princeton
- Agency: Marshall, Gerstein & Borun LLP
- Main IPC: C12Q1/34
- IPC: C12Q1/34 ; G01N33/573
Abstract:
The present application provides a method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human sirtuin 4 (SIRT4) comprising measuring a level of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the cell. The present application also demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.
Public/Granted literature
- US20210318312A1 SIRT4 Lipoamidase Activity and Uses Thereof Public/Granted day:2021-10-14
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