公开(公告)号：US10813934B2

公开(公告)日：2020-10-27

申请号：US15187269

申请日：2016-06-20

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Emre Koyuncu ,  Ileana M. Cristea

IPC: A61K31/40 ,  A61K31/35 ,  A61K31/12 ,  A61K31/497 ,  A61K31/47 ,  A61K31/513 ,  A61K31/167 ,  A61K31/17 ,  A61K31/404 ,  A61K45/06 ,  A61K31/366 ,  A61K31/496 ,  A61K31/498

Abstract: Agents and methods of inhibiting or improving the growth of viruses are provided.

公开(公告)号：US20160296523A1

公开(公告)日：2016-10-13

申请号：US15187269

申请日：2016-06-20

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Emre Koyuncu ,  Ileana M. Cristea

IPC: A61K31/513 ,  A61K31/17 ,  A61K31/366 ,  A61K31/498 ,  A61K31/496 ,  A61K31/404 ,  A61K45/06 ,  A61K31/167

Abstract: Agents and methods of inhibiting or improving the growth of viruses are provided.

Abstract translation: 提供了抑制或改善病毒生长的药剂和方法。

公开(公告)号：US20210380952A1

公开(公告)日：2021-12-09

申请号：US17381930

申请日：2021-07-21

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Emre Koyuncu ,  Ileana M. Cristea ,  Thomas Shenk

IPC: C12N7/00 ,  C12N5/00

Abstract: A method is provided to improve virus production is an infected host cell by culturing the infected cell in an effective amount of alpha-ketoglutarate.

公开(公告)号：US10300130B2

公开(公告)日：2019-05-28

申请号：US15223980

申请日：2016-07-29

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Dai Wang

IPC: C07K14/11 ,  A61K39/245 ,  A61K39/12 ,  C12N7/00 ,  A61K39/145 ,  A61K39/00

Abstract: Methods of increasing diversity in cytomegalovirus vaccines through the selection of cell type in which the virus is propagated, and the use of cytomegalovirus produced by those methods in the development of vaccine compositions, are disclosed. Vaccine compositions comprising CMV isolated from epithelial cells are also disclosed.

公开(公告)号：US20140113353A1

公开(公告)日：2014-04-24

申请号：US14112824

申请日：2012-04-18

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Thomas Shenk ,  Emre Koyuncu ,  Joshua D. Rabinowitz

IPC: C12N7/00

CPC classification number: C12N7/00 ,  C12N2710/16151 ,  C12N2710/16751

Abstract: A method for improving virus production in a host cell infected with the virus is provided.

Abstract translation: 提供了用于改善感染病毒的宿主细胞中病毒产生的方法。

公开(公告)号：US11726088B2

公开(公告)日：2023-08-15

申请号：US16991429

申请日：2020-08-12

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Thomas Shenk ,  Todd M. Greco ,  Ileana M. Cristea ,  Rommel A. Mathias ,  Adam Oberstein

IPC: C12Q1/34 ,  G01N33/573

CPC classification number: G01N33/573 ,  C12Q1/34 ,  G01N2333/90212 ,  G01N2333/91057 ,  G01N2500/00

Abstract: The present application provides a method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human sirtuin 4 (SIRT4) comprising measuring a level of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the cell. The present application also demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.

公开(公告)号：US11129891B2

公开(公告)日：2021-09-28

申请号：US16382125

申请日：2019-04-11

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Dai Wang

IPC: A61K39/245 ,  A61K39/12 ,  C07K14/005 ,  C07K16/08 ,  C12N7/00 ,  C12Q1/02 ,  A61K39/00

Abstract: Immunogenic compositions and prophylactic or therapeutic vaccines for use in protecting and treating against human cytomegalovirus (CMV) are disclosed. Subunit vaccines comprising a human CMV protein complex comprising pUL128 or pUL130, and nucleic acid vaccines comprising at least one nucleic acid encoding a CMV protein complex comprising pUL128 or pUL130 are described. Also disclosed are therapeutic antibodies reactive against a CMV protein complex comprising pUL128 or pUL130, as well as methods for screening compounds that inhibit CMV infection of epithelial and endothelial cells, methods for immunizing a subject against CMV infection, methods for determining the capability of neutralizing antibodies to inhibit CMV infection of cell types other than fibroblasts, and methods of diminishing an CMV infection.

公开(公告)号：US20190094225A1

公开(公告)日：2019-03-28

申请号：US15106932

申请日：2015-01-15

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Todd M. Greco ,  IIeana M. Cristea ,  Rommel A. Mathias ,  Adam Oberstein

IPC: G01N33/573

CPC classification number: G01N33/573 ,  C12Q1/34 ,  G01N2333/90212 ,  G01N2333/91057 ,  G01N2500/00

Abstract: The present application demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.

公开(公告)号：US20180298348A1

公开(公告)日：2018-10-18

申请号：US16013619

申请日：2018-06-20

Applicant: The Trustees of Princeton University

Inventor： Emre Koyuncu ,  Ileana M. Cristea ,  Thomas Shenk

IPC: C12N7/00 ,  C12N5/00

Abstract: The present disclosure provides a method to enhance the production of virus in cultured fibroblasts by supplementing the cells with a TCA cycle intermediate, aketoglutarate, or a derivative thereof, wherein virus production is enhanced compared to the same method canied out in the absence of a-ketoglutarate or the derivative thereof. In view of the art, the method provides an unexpected improvement on methods routinely practiced.

公开(公告)号：US20170014504A1

公开(公告)日：2017-01-19

申请号：US15223980

申请日：2016-07-29

Applicant: The Trustees of Princeton University

Inventor： Thomas Shenk ,  Dai Wang

IPC: A61K39/245 ,  C12N7/00

CPC classification number: A61K39/245 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/54 ,  C12N7/00 ,  C12N2710/16134 ,  C12N2710/16151 ,  C12N2710/16164

Abstract: Methods of increasing diversity in cytomegalovirus vaccines through the selection of cell type in which the virus is propagated, and the use of cytomegalovirus produced by those methods in the development of vaccine compositions, are disclosed. Vaccine compositions comprising CMV isolated from epithelial cells are also disclosed.

Abstract translation: 公开了通过选择扩增病毒的细胞类型增加巨细胞病毒疫苗多样性的方法，以及通过这些方法产生的巨细胞病毒在开发疫苗组合物中的应用。 还公开了包含从上皮细胞分离的CMV的疫苗组合物。