Systems and methods for fast autotuning of industrial fixed vision cameras are disclosed herein. An example method includes modifying illumination settings of a camera until a brightness value for captured image data reaches a minimum value; modifying a focus level of the camera to determine a range of camera focus levels for successfully decoding barcodes; configuring the camera to operate using a midpoint focus value of the range; modifying illumination settings of the camera until the brightness associated with the captured image data reaches an optimal value; and modifying the focus level of the camera, within the range, to determine an optimal focus level at which sharpness for the captured image is optimized; and capturing, by the camera, operating at the determined optimal focus level and with illumination settings for the optimal threshold brightness value, image data associated with a new target object affixed with a new barcode.