A calibration assembly may be used with an endoscopic imaging system having a field of view. The calibration assembly may comprise an interface configured for constraining engagement with the endoscopic imaging system and a target coupled with the interface so as to be within the field of view. The target may include a plurality of calibration features. The calibration assembly may be reconfigurable from a first spatial arrangement between the target and the endoscopic imaging system to a second spatial arrangement between the target and the endoscopic imaging system. The calibration assembly may also comprise a processor configured to obtain, from the endoscopic imaging system, first and second images of at least some of the calibration features of the target at the first and second spatial arrangements. The processor may also generate calibration data for the endoscopic imaging system using the first and second images.