A preparation method and a device for a seawater desalination-seawater extraction uranium membrane lining are provided. The preparation method is as follow: the lining is configured as a finished product for standby through the following process, including cleaning, drying, restoration of circular, generating burrs, fixing the length of burrs; the device includes the first module, the second module, the third module, the fourth module and the fifth module. The present invention has the advantages of simple operation, short time, low cost and obvious treatment effect, the bonding strength between the separation function layer and the lining is enhanced, and the separation function layer is not easy to fall off, the physical damage resistance is greatly increased, it is not easy to fall off and the initial bubble point pressure of the prepared enhanced film is high.