Provided is a display substrate. The display substrate includes: a base substrate including a display region and a non-display region surrounding the display region; a gate drive circuit disposed in the non-display region; a plurality of first signal lines disposed in the peripheral region and connected to the gate drive circuit; and a plurality of second signal lines disposed in the non-display region and connected to the gate drive circuit; wherein each of the first signal line and the second signal line is configured to supply a signal to the gate drive circuit, and a frequency of the signal supplied by the first signal line is lower than a frequency of the signal supplied by the second signal line.