A collision reaction pool ion acceleration apparatus which has extremely low crosstalk. The apparatus comprises an apparatus body, a vacuum chamber, a first tube bundle channel and a second tube bundle channel. The vacuum chamber is fixedly connected to the interior of the apparatus body; the other end of the interior of the apparatus body is fixedly connected to a first insulation seat. A collision chamber is embeddedly connected to the inside the first insulation seat, and a high-frequency electrode quadrupole lens is fixedly connected to two sides of the collision chamber. When charged ions enter the collision chamber, the high-frequency electrode quadrupole lens focuses on the charged ions, so that the incoming charged ions form a new motion trajectory in the collision chamber, and the charged ions are easily separated from the collision chamber, thereby increasing the working efficiency.